Burn Injury Inhibits Neutrophil Chemotaxis in Microfluidic Devices
نویسنده
چکیده
Thermal injury can trigger a fulminant inflammatory cascade that can lead to sepsis and death. Although several prior studies have documented impairments in neutrophil chemotaxis concurrent with these post-burn inflammatory changes, cumbersome assays have prohibited further exploration of the significance of these findings. To address this need, we developed a microfluidic device that is simple to operate and allows for chemotaxis measurements at single-cell resolution. Our study on neutrophils from eight burn patients revealed impaired migration speed starting at 24 hours after injury, reaching a minimum at 72-120 hours, and potentially serving as an early indicator for concurrent infections.
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Burn Injury Reduces Neutrophil Directional Migration Speed in Microfluidic Devices
Thermal injury triggers a fulminant inflammatory cascade that heralds shock, end-organ failure, and ultimately sepsis and death. Emerging evidence points to a critical role for the innate immune system, and several studies had documented concurrent impairment in neutrophil chemotaxis with these post-burn inflammatory changes. While a few studies suggest that a link between neutrophil motility a...
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